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Image Search Results
Journal: Nature protocols
Article Title: Ex vivo perfusion of mid-to-late-gestation mouse placenta for maternal-fetal interaction studies during pregnancy
doi: 10.1038/nprot.2012.144
Figure Lengend Snippet: Overview of the ex vivo perfusion system. (a) BubbleStop syringe warmer (SW); Teflon valve box (VB); ValveLink controller (VC); low-flow peristaltic pump (LFP); medium-flow peristaltic pump (MFP); in-line solution heater (SH); incubation chamber (IC); temperature controllers (TC); ultralow-flow peristaltic pumps (ULP). (b) Layout and function of the components of the ex vivo perfusion system. (c) Polyamide-coated, fused silica capillaries of various diameters inserted into Micro-Renathane tubing of 0.033-inch i.d. and sealed with multiple applications of cyanoacrylate.
Article Snippet: BubbleStop syringe warmer (Automate Scientific, cat. no. 10-4-60-G) ValveLink 8.2 (Automate Scientific, cat. no. 01-18) Teflon valve box (Automate Scientific, cat. no. 02-01-02) Vacuum bottle, 4 liters (VWR, cat. no. 16331-100) Teflon tubing, 1/16th inch (Automate Scientific, cat. no. bi-20202275) Syringes, 60 ml (Automate Scientific, cat. no. 06-60C) Ultralow-flow peristaltic pump (×2) (Instech, cat. no. P720/10k) Low-flow peristaltic pump (Instech, cat. no. P720) USB interface for perfusion pumps (Bioptechs, cat. no. 13161603-13) C-Flex tubing, 0.020-inch inner diameter (i.d.) (Instech, cat. no. P720/TS-BS020SBS) C-Flex tubing, 0.031-inch i.d. (Instech, cat. no. P720/TS-BS031SBS) Cyanoacrylate glue (Office Depot, cat. no. 903720) Micro-Renathane tubing, 0.033 inch (Braintree Scientific, cat. no. MRE-033-CL-100) Silica tubing, 10 m, 090 μm (Polymicro, cat. no. TSP020090) Silica tubing, 10 m, 105 μm (Polymicro, cat. no. TSP040105) Silica tubing, 10 m, 150 μm (Polymicro, cat. no. TSP075150) Silica tubing, 10 m, 200 μm (Polymicro, cat. no. TSP100200) Micro clamp (×5), 7 mm (Fine Science Tools, cat. no. 00396-01) Micro clamp, 11 mm (Fine Science Tools, cat. no. 00398-02)
Techniques: Ex Vivo, Incubation
Journal: Nature protocols
Article Title: Ex vivo perfusion of mid-to-late-gestation mouse placenta for maternal-fetal interaction studies during pregnancy
doi: 10.1038/nprot.2012.144
Figure Lengend Snippet: Extraction and isolation of an E18 mouse placenta. (a) Once the mouse has been euthanized and the peritoneal cavity opened, splay the uterine horns out to reveal the uterine artery for excision. (b) Remove the embryos surrounding the placenta of interest and preserve 4–5 mm of uterine artery for cannulation. (c) Once separated, immediately transfer the uterine segment containing the placenta of interest to the incubation chamber. (d) At this point, open the amniotic sac and separate the placenta from the embryo, severing the umbilical cord as near to the embryo as possible. All animal experiments have been conducted in accordance with the National Institutes of Health Animal Use Guidelines and approved by the Institutional Animal Care and Use Committee at The University of Southern California.
Article Snippet: BubbleStop syringe warmer (Automate Scientific, cat. no. 10-4-60-G) ValveLink 8.2 (Automate Scientific, cat. no. 01-18) Teflon valve box (Automate Scientific, cat. no. 02-01-02) Vacuum bottle, 4 liters (VWR, cat. no. 16331-100) Teflon tubing, 1/16th inch (Automate Scientific, cat. no. bi-20202275) Syringes, 60 ml (Automate Scientific, cat. no. 06-60C) Ultralow-flow peristaltic pump (×2) (Instech, cat. no. P720/10k) Low-flow peristaltic pump (Instech, cat. no. P720) USB interface for perfusion pumps (Bioptechs, cat. no. 13161603-13) C-Flex tubing, 0.020-inch inner diameter (i.d.) (Instech, cat. no. P720/TS-BS020SBS) C-Flex tubing, 0.031-inch i.d. (Instech, cat. no. P720/TS-BS031SBS) Cyanoacrylate glue (Office Depot, cat. no. 903720) Micro-Renathane tubing, 0.033 inch (Braintree Scientific, cat. no. MRE-033-CL-100) Silica tubing, 10 m, 090 μm (Polymicro, cat. no. TSP020090) Silica tubing, 10 m, 105 μm (Polymicro, cat. no. TSP040105) Silica tubing, 10 m, 150 μm (Polymicro, cat. no. TSP075150) Silica tubing, 10 m, 200 μm (Polymicro, cat. no. TSP100200) Micro clamp (×5), 7 mm (Fine Science Tools, cat. no. 00396-01) Micro clamp, 11 mm (Fine Science Tools, cat. no. 00398-02)
Techniques: Extraction, Isolation, Incubation